Plasmid_Backbone

Part:BBa_K1138000:Design

Designed by: Rachit Jain   Group: iGEM13_UGA-Georgia   (2013-09-06)


pAW42-gs (ugaiGEM2)


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 1553
    Illegal XbaI site found at 1883
    Illegal XbaI site found at 1892
    Illegal SpeI site found at 6597
    Illegal PstI site found at 2421
  • 12
    INCOMPATIBLE WITH RFC[12]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 1553
    Illegal SpeI site found at 6597
    Illegal PstI site found at 2421
    Illegal NotI site found at 2608
  • 21
    INCOMPATIBLE WITH RFC[21]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 1553
    Illegal BglII site found at 1870
    Illegal BamHI site found at 2402
  • 23
    INCOMPATIBLE WITH RFC[23]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 1553
    Illegal XbaI site found at 1883
    Illegal XbaI site found at 1892
    Illegal SpeI site found at 6597
    Illegal PstI site found at 2421
  • 25
    INCOMPATIBLE WITH RFC[25]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 1553
    Illegal XbaI site found at 1883
    Illegal XbaI site found at 1892
    Illegal SpeI site found at 6597
    Illegal PstI site found at 2421
    Illegal AgeI site found at 538
    Illegal AgeI site found at 2444
    Illegal AgeI site found at 2620
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal BsaI site found at 4778
    Illegal BsaI.rc site found at 3161
    Illegal BsaI.rc site found at 3490
    Illegal SapI site found at 6442


Design Notes

Codon optimized for expression in Methanococcus.


Source

The gs gene was obtained from Ocimum basilicum.

References